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primary polyclonal antibody anti topoisomerase ii alpha

primary polyclonal antibody anti topoisomerase ii alpha

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(PDF) Anti-topoisomerase II alpha autoantibodies in

We have previously detected autoantibodies against topoisomerase II alpha (anti-topo II alpha) in sera from patients with idiopathic pulmonary fibrosis.To determine whether anti-topo II alpha is also present in systemic sclerosis (SSc) patients with(PDF) Both alpha and beta isoforms of mammalian DNA The polyclonal anti-topoisomerase II antibody identified a M r 170,000 protein in the Ptk1 cell extract.The antibody to topoisomerase II recognized a band with mobility of M r 180,000 in both HeLa cell and Ptk1 cell extracts.This band corresponds to full-length topoisomerase II.

(PDF) DNA topoisomerase II interacts with Lim15/Dmc1 in

Nucleic Acids Research,2005,Vol.33,No.18 5811 Co-immunoprecipitation D-loop assays Rabbit anti-CcTopII polyclonal antibodies,rabbit anti- D-loop reactions were performed essentially as described CcLim15 polyclonal antibodies and control rabbit serum previously (42).(PDF) HMGB1 and HMGB2 proteins up-regulate cellular HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase II.pdf. with the primary antibody,the. and immunodetection with polyclonal anti-pRb antibody as detailed in

Alpha,beta-unsaturated lactones 2-furanone and 2

Sep 12,2013·Anti-topo II polyclonal antibody CT was raised in rabbits to recombinant topo II (C-terminal fragment) and detected the isoform of topo II.For topo I,a polyclonal human antibody (2012,Topogen) was used.Antibodies were diluted in PBS containing 0.1% Tween 20 and 1% BSA (bovine serum albumin).CT (topo II) was used at a 1:50 dilution Anti Topoisomerase II Alpha Antibody (PrecisionAb Rabbit anti Human topoisomerase II alpha antibody recognizes the DNA topoisomerase II alpha,also known as DNA gyrase or DNA topoisomerase (ATP-hydrolyzing).TOP2A gene encodes a DNA topoisomerase,an enzyme that controls and alters the topologic states of DNA

Anti-Fas Antibody Boster PA1119 product information

May 05,2019·FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 ug/mL.The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).Anti-Fas antibody,PA1119,IHC(P).IHC(P) Rat Spleen Tissue Lysate Figure 5.IHC analysis of FAS using anti-FAS antibody (PA1119).Anti-Histone H3 polyclonal antibody (CABT-LM00434 Antibodies.Primary Antibodies; Secondary Antibodies; Control Antibodies; Hybridomas.ELISA Kits. Anti-Histone H3 polyclonal antibody (CABT-LM00434) Rabbit Anti-Human Histone H3 polyclonal antibody for WB,IHC,IF Patton,EE; et al.Topoisomerase II alpha Is Required for Embryonic Development and Liver Regeneration in Zebrafish

Anti-Histone H3 polyclonal antibody (CABT-LM00434

Dovey,M; Patton,EE; et al.Topoisomerase II alpha Is Required for Embryonic Development and Liver Regeneration in Zebrafish.MOLECULAR AND CELLULAR BIOLOGY 29:3746-3753(2009).MOLECULAR AND CELLULAR BIOLOGY 29:3746-3753(2009).Anti-Human NFE2L2 Antibody raised in Rabbit- CUSABIOThis antibody is a rabbit polyclonal antibody against NFE2L2.It has been selected for its ability to recognize NFE2L2 in Human,Mouse,Rat. NRF2 protein acetylation contributes to ABCC1-mediated chemoresistance and glutathione-mediated survival in acquired topoisomerase II poison-resistant cancer cells. The difference between primary

Anti-MIB1 monoclonal antibody (CABT-B1367) - Creative

Lynch,BJ; Guinee,DG; et al.Human DNA topoisomerase II-alpha A new marker of cell proliferation in invasive breast cancer.HUMAN PATHOLOGY 28:1180-1188(1997).HUMAN PATHOLOGY 28:1180-1188(1997).Online InquiryAnti-TOP2A Antibody,Rabbit Polyclonal,101145-T32 Sino It has been reported that Topoisomerase II alpha (TOP2A) could induce tumor development and progression in many cancer types.The up-regulation of TOP2A was significantly correlated with tumor metastasis and shorter survival in patients with pancreatic cancer.

Anti-Transglutaminase type II Antibody 06-471

More>> This Anti-Transglutaminase type II Antibody is validated for use in IP,WB for the detection of Transglutaminase type II.Less<< Anti-Transglutaminase type II Antibody MSDS (material safety data sheet) or SDS,CoA and CoQ,dossiers,brochures and other available documents.Anti-Transgutaminase 2/TGM2 Antibody UCB-Bioproducts Amrubicin Topoisomerase II inhibitor; Description A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TGM2 .This antibody is tested and proven to work in the following applications Anti-HIF-2-alpha/EPAS1 Antibody PA1129-2 BosterBio 100ug/vial EUR 294 Transglutaminase 2,Tissue (TGM2) Antibody

Anti-human Antibodies Gentaur

Polyclonal antibodies anti- humand protein are perfect for Western Blotting or Frozen IHC Immuno Histo Chemistry.Mouse Monoclonal Clones are used in Flow cytometry coupled to FITC.The Gentaur Antibodies are supplied in 100 ug / 100ul and are used in a dilution of 1/500 to 1/5000 Secondairy antibodies anti Rabbit our anti Mouse are available too.Antibodies,Proteins,Kits and Reagents for Life Science Abcam,the leading supplier of protein research tools to life scientists.Discover more from a range of 118,000 antibodies,kits,proteins and other reagents

Both and ß Isoforms of Mammalian DNA Topoisomerase II

In HeLa cell extracts,antibodies to topoisomerase II,both monoclonal and polyclonal,recognized a single band of M r 170,000.The monoclonal antibody to topoisomerase II recognizes only protein from primate cells,so it is not useful for Ptk1 cells.The polyclonal anti-topoisomerase II antibody identified a M r 170,000 protein in the Ptk1 Both and ß Isoforms of Mammalian DNA Topoisomerase II In HeLa cell extracts,antibodies to topoisomerase II,both monoclonal and polyclonal,recognized a single band of M r 170,000.The monoclonal antibody to topoisomerase II recognizes only protein from primate cells,so it is not useful for Ptk1 cells.The polyclonal anti-topoisomerase II antibody identified a M r 170,000 protein in the Ptk1 cell extract.

Clinical significance of topoisomeraseII expression in

Jan 03,2020·Topoisomerase II alpha (TopoII) is a nuclear enzyme that is upregulated in proliferating cells. Immunohistochemical staining was performed as previously described 23 using antiTopoII,an affinity purified rabbit polyclonal antibody (ab180393; Abcam,Cambridge,UK; 1:100 dilution).Cells with stained nuclei were defined as having Conference Proceedings 4 th International Conference on Dec 10,2019·The aim of this work is to detect in female blood anti-idiotypic antibodies from alpha-fetoprotein,which selectively suppress the synthesis of anti-bodies against embryo alpha-fetoprotein.Methodology.The work used the blood of the woman in childbirth.The amount of 5 ml blood was placed in a thermostat at a temperature of 36 °C until a clot

Cytokeratin 5 6 Bioz Ratings For Life-Science Research

Anti CK 5 CK 6 Cytokeratin 5 Cytokeratin 6 Rabbit Monoclonal Antibody Clone RM341 tested in WB IHC reactive to Human Buy from Supplier ck5 6 (Thermo Fisher) 94.Thermo Fisher ck5 6 Ck5 6,supplied by Thermo Fisher,used in various techniques.Bioz Stars score 94/100,based onDCTD Gene - GeneCards DCTD Protein DCTD AntibodyApr 05,2021·DCTD (DCMP Deaminase) is a Protein Coding gene.Diseases associated with DCTD include Aspartylglucosaminuria and Granulomatosis With Polyangiitis.Among its related pathways are Metabolism of nucleotides and Metabolism.Gene Ontology (GO) annotations related to this gene include hydrolase activity and dCMP deaminase activity.

DNA topoisomerase I and II expression in drug

Sep 03,2002·A small number of testicular germ cell tumours are refractory to current chemotherapy regimens.DNA topoisomerase I is the target for several new drugs and aDNA topoisomerase I and II expression in drug Sep 03,2002·A small number of testicular germ cell tumours are refractory to current chemotherapy regimens.DNA topoisomerase I is the target for several new drugs and a

Detection of Human Topoisomerase II in Cell Lines and

We report five novel monoclonal antibodies (Ki-S1,Ki-S4,Ki-S6,Ki-S7,and Ki-S8) reactive with a proliferation-related nuclear antigen.In immunoprecipitation and Western blot experiments using cEstablishment Registration Device Listingimmunohistochemistry antibody assay,estrogen receptor - CONFIRM anti-Estrogen Receptor (ER) (SP1) Rabbit Monoclonal Primary Antibody Manufacturer immunohistochemistry assay,antibody,anaplastic lymphoma kinase - VENTANA ALK (D5F3) CDx Assay

Evaluation of the prognostic role of centromere 17 gain

·Despite advances,a significant proportion of patients with Hodgkins lymphoma (HL) will not respond or will relapse.We demonstrated that up to seven infusions of 90Y-daclizumab,an anti-CD25directed monoclonal antibody,provided responses in 50% of patients with relapsed HL.The daclizumab was directed primarily not at tumor cells themselves but toward nonmalignant T cellsEvaluation of the prognostic role of centromere 17 gain ·Despite advances,a significant proportion of patients with Hodgkins lymphoma (HL) will not respond or will relapse.We demonstrated that up to seven infusions of 90Y-daclizumab,an anti-CD25directed monoclonal antibody,provided responses in 50% of patients with relapsed HL.The daclizumab was directed primarily not at tumor cells themselves but toward nonmalignant T cells

Evaluation of the prognostic role of centromere 17 gain

Mar 28,2013·One such gene is the topoisomerase II alpha gene (TOP2A),which is located ~700 kb telomerically to HER2 and encodes the alpha isozyme of the human topoisomerase II.In general,topoisomerases are responsible for transcription,replication and chromosome condensation and segregation during cell division [ 31 ,32 ].Gapdh Antibody Cell Signaling Technology Inc Biozrabbit lats1 polyclonal antibody against microtubule-associated protein 1 light chain 3 Maternal topoisomerase II alpha,not topoisomerase II blocked,and subjected to overnight primary antibody incubation with anti-ETO C-20 (Santa Cruz),anti-Brn3a MAB1585 (Chemicon),anti -actin (Sigma),anti-GAPDH,anti-Tubulin or anti-HA

HMGB1 and HMGB2 proteins up-regulate cellular expression

INTRODUCTION.DNA topoisomerase II (topo II) is an essential and ubiquitous enzyme for proliferation of eukaryotic cells ().It can alter the topological state of DNA and untangle DNA knots and catenanes (interlocked rings) via ATP-dependent passing of an intact double helix through a transient double-stranded break generated in a separate DNA segment,followed by religation and enzyme turnover ().Her-2 expression in cutaneous eccrine and apocrine Nov 19,2003·Using a rabbit polyclonal antibody directed against a 15-amino-acid sequence of the human neu gene,substantial staining was noted in the secretory cells

High expression of Bcl-2 predicts poor outcome in

UK),rabbit anti-human polyclonal antibody against Topo II (Topoisomerase II alpha Ab-4; dilution,1:200,Thermo Fisher Scientific,Fremont,CA,USA),and -tubulin (Rab-bit Anti-Human Tubulin Polyclonal Antibody; dilution,1:200,Spring bioscience,Pleasanton,CA,USA).After several rinses in phosphate-buffered saline,the sections were Human TOP2B Antibody AF6348 RD SystemsTOP2B (DNA Topoisomerase II beta ) is a 180185 kDa member of the type IIA subfamily,topoisomerase family of molecules.It is ubiquitously expressed,and represents the larger of two known topoisomerases (the 2A/ alpha -form being 170 kDa in size).TOP2A is essential for cell division,while TOP2B is active postmitotically.

IHC Primary Antibodies - IHC ISH - Leica Biosystems

IHC Primary Antibodies Create high-quality IHC slides with Novocastra primary antibodies.Developed in-house,and backed by 20 years of IHC stain development experience,these robust antibodies have been optimized for automated and manual applications.IHC Primary Antibodies - IHC ISH - Leica BiosystemsIHC Primary Antibodies Create high-quality IHC slides with Novocastra primary antibodies.Developed in-house,and backed by 20 years of IHC stain development experience,these robust antibodies have been optimized for automated and manual applications.

Images of Primary Polyclonal antibody Anti Topoisomerase II

imagesRelated searches for primary polyclonal antibody anti topoipolyclonal antibody therapypolyclonal antibody preparationpolyclonal antibody drugmonoclonal vs polyclonal antibodiesregeneron's polyclonal antibody12345Next(PDF) Anti-topoisomerase II alpha autoantibodies in We have previously detected autoantibodies against topoisomerase II alpha (anti-topo II alpha) in sera from patients with idiopathic pulmonary fibrosis.To determine whether anti-topo II alpha is also present in systemic sclerosis (SSc) patients withPolyclonal Antibody to Collagen Type II Alpha 1 (COL2a1 Figure.Western Blot; Sample Recombinant COL2a1,Mouse.Western Blot; Sample Mouse Serum; Primary Ab 1.5µg/ml Rabbit Anti-Mouse COL2a1 Antibody Second Ab 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog SAA544Rb19)

RECQL5 cooperates with Topoisomerase II alpha in DNA

Oct 19,2011·The following antibodies were used as described in the Results section- Primary RECQL5 (1:200,rabbit),Topoisomerase II (Santa Cruz Biotechnology; 1:200,mouse).Secondary Donkey anti-rabbit AlexaFluor 488 at 1:1000 dilution and donkey antiRECQL5 cooperates with Topoisomerase II alpha in DNA Oct 19,2011·Topoisomerase II is the molecular target for many clinically useful anti-neoplastic drugs (18,19).Studies have also implicated Topoisomerase II as a potential prognostic marker in tumors (2022).Interestingly,SGS1,the RecQ homolog in yeast,interacts with Topoisomerase II to faithfully segregate chromosomes (23).

RECQL5 cooperates with Topoisomerase II alpha in DNA

The following antibodies were used as described in the Results section- Primary RECQL5 (1:200,rabbit),Topoisomerase II (Santa Cruz Biotechnology; 1:200,mouse).Secondary Donkey anti-rabbit AlexaFluor 488 at 1:1000 dilution and donkey anti-mouse AlexaFluor 647,1:1000 dilutions.RESEARCH Open Access P53,MAPK,topoisomerase IIBackground We investigated the immunohistochemical expression of p53,MAPK,topoisomerase II alpha (topoII alpha) and Ki67 in ovarian serous carcinomas (OSCs) along with mutational analysis for KRAS and BRAF.Methods Eighty one cases of OSCs were reviewed and examined immunohistochemically using antibodies against p53,MAPK,topoII alpha and Ki67.

RESEARCH Open Access P53,MAPK,topoisomerase II

P53,MAPK,topoisomerase II alpha and Ki67 immunohistochemical expression and KRAS/BRAF mutation in ovarian serous carcinomas with rabbit polyclonal antibody,pTEpY,which specifically reacts with phosphorylated (active) MAPK (Promega, created by omission of the primary antibody.RPL41 Antibody - DF9136 - affinity_biosciencesPolyclonal.Purification Interacts with the beta subunit of protein kinase CKII and stimulates phosphorylation of DNA topoisomerase II alpha by CKII.Similarity Secondary antibody Goat Anti-Rabbit IgG (H+L) HRP Goat Anti-Rabbit IgG (H+L) AP

RPL41 antibody anti RPL41 antibody Biorbyt

Rabbit polyclonal antibody to RPL41 also known as HG12,whose expression can be detected in the tongue but is highly expressive in the tonsils and amniotic fluid.It is found in the cytosolic large ribosomal subunit.This protein interacts with the beta subunit of protein kinase CK2 and stimulates phosphorylation of DNA topoisomerase II alpha Reagents>Chromatin / Epigenetic / Cell cycleKDM1/LSD1 Rabbit Polyclonal Antibody (KO Validated) 50l RMB550.00 Add to cart AF5635 Mono-Methyl-Histone H3 (Lys4) Rabbit Polyclonal Antibody 50l RMB550.00 Add to cart Topoisomerase II alpha Rabbit Monoclonal Antibody 50l RMB658.00 Add to cart AF1606 Cyclin B1 Rabbit Monoclonal Antibody 50l RMB658.00 Add to cart:

Related searches for primary polyclonal antibody anti topoi

polyclonal antibody therapypolyclonal antibody preparationpolyclonal antibody drugmonoclonal vs polyclonal antibodiesregeneron's polyclonal antibodySome results are removed in response to a notice of local law requirement.For more information,please see here.Related searches for primary polyclonal antibody anti topoipolyclonal antibody therapypolyclonal antibody preparationpolyclonal antibody drugmonoclonal vs polyclonal antibodiesregeneron's polyclonal antibodySome results are removed in response to a notice of local law requirement.For more information,please see here.Previous123456Next(PDF) Immunohistochemical study of topoisomerase II- Higher levels of topoisomerase II-alpha expression were strongly related to higher tumour grade,larger tumour size,nodal status,and the presence of distant metastases at diagnosis.

TOP2A antibody 14 products in Validated Antibody

Jan 16,2021·Immunohistochemical analysis of paraffin-embedded SAS Xenograft using Topoisomerase II alpha (PA5-27248) antibody at 1:500 dilution.Western Blot analysis of Topoisomerase II alpha using anti-Topoisomerase II alpha Polyclonal Antibody (PA5-27248),in H1299 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:3000.quantity 100µLTOP2A-Specific Antibody 20233-1-AP ProteintechTOP2A-Specific antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB),Immunoprecipitation (IP),Immunohistochemistry (IHC),Immunofluorescence (IF),Enzyme-linked Immunosorbent Assay (ELISA) applications.This antibody reacts with human samples.Cat.No.20233-1-AP.KD/KO Validated.

TOPOISOMERASE II ALPHA EXPRESSION AND

Abstract Topoisomerase II is ATP dependent enzyme that catalyzes DNA strand passage,the pivotal process in replication,transcription,recombination etc.As part of this breakage and religation process the intermediate generated is a cleavable complex between DNA and topoisomerase II.Testosterone Polyclonal antibody 100ug UCB-Bioproducts Amrubicin Topoisomerase II inhibitor; Description A Rabbit polyclonal antibody against General Testosterone (Testo).This antibody is labeled with Biotin.Testosterone (Testo) Polyclonal Antibody (General),Cy3 Description Primary antibody against Testosterone(4E1G2),CF405S conjugate,Concentration 0.1mg/mL.

TopBP1 Antibody (AF7120) Novus Biologicals

Sheep Polyclonal Anti-TopBP1 Antibody.Validated IHC.Tested Reactivity Human.100% Guaranteed.Topoisomerase I Antibody T8N - laebioTopoisomerase I Antibody T8N (Catalog # A P005) Specificity Rabbit polyclonal antibody against Human topoisomerase I.Antigen used for immunizations Prokaryotic recombinant protein corresponding to the N-terminal 2-11 amino acid region of the human topoisomerase I [access no P11387] molecule.

Topoisomerase II alpha gene amplification is a favorable

Oct 23,2012·The topoisomerase II alpha gene (TOP2A) is located telomerically to HER2 at 17q21-q22 and encodes for topoisomerase II alpha (TopoIIa),a 170-kd cell cycle regulated protein.The TOP2A gene is considered to be within the HER2 amplicon[ 8 ],although it is not included in the smallest region of amplification[ 9 ] and may follow a different Topoisomerase II represses transcription by enforcing Apr 13,2021·Rabbit monoclonal antibody against Topoisomerase II Alpha (Topo IIa)(EP1102Y) Abcam Rabbit Anti-Histone H3,phospho (Ser10) Mitosismarker Polyclonal antibody,Unconjugated Millipore Cat# 06-570 927-40000) for 1 hour and then probed with required primary antibodies for 2 hours.Membranes were washed with 0.1% Tween-20 - Odyssey

anti-Homo sapiens (Human) SMC6 Antibody raised in

The SMC5/6 complex physically interacts with the DNA topoisomerase II alpha (TOP2A).PMID 27792189 results uncover a novel role for the Smc5/6 complex as a restriction factor selectively blocking extrachromosomal DNA transcription; by destroying this complex,HBx relieves the inhibition to allow productive hepatitis B virus gene expression anti-TOP2 antibody (AA 1079-1328) Product No.anti-DNA Topoisomerase II (TOP2) (AA 749-987) antibody Primary Antibody TOP2 Reactivity Mouse IF/ICC,IHC,IP,WB Host Rabbit Polyclonal unconjugated

anti-TOP2A,Polyclonal,Novus Biologicals 0.1mL; Unlabeled

The immunogen recognized by this antibody maps to a region between residue 1500 and the C-terminus (residue 1531) of human Topoisomerase (DNA) II Alpha using the numbering given in entry NP_001058.2 (GeneID 7153).Purification Method Immunogen affinity purified Regulatory Status RUO Storage Requirements Store at 4C.Do not freeze.Monoclonal or Polyclonalanti-Topoisomerase (DNA) II Binding Protein 1 Anticorpsanti-Topoisomerase (DNA) II Binding Protein 1 (TOPBP1) (AA 1327-1435) anticorps Primary Antibody TOPBP1 Reactivité Humain ELISA,WB Hôte Souris Polyclonal unconjugated

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